CBSE Grade-12 Biology | 9. Biotechnology : Principles and Processes by Pavan | Learn Smarter
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9. Biotechnology : Principles and Processes

9. Biotechnology : Principles and Processes

Biotechnology integrates natural sciences and organisms to create products beneficial to humans, marking a shift towards genetic engineering and bioprocess engineering. Key milestones include the development of recombinant DNA technology, which allows for the manipulation of genes and large-scale production of proteins using microorganisms. This chapter discusses the principles, tools, and processes of biotechnology, paving the way for advancements in health and agriculture.

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  1. 9
    Biotechnology : Principles And Processes

    This section introduces biotechnology as a pivotal field that leverages...

  2. 9.1
    Principles Of Biotechnology

    This section covers the fundamental principles behind biotechnology,...

  3. 9.2
    Tools Of Recombinant Dna Technology

    The section discusses the essential tools necessary for recombinant DNA...

  4. 9.2.1
    Restriction Enzymes

    Restriction enzymes are molecular scissors that cut DNA at specific...

  5. 9.2.2
    Cloning Vectors

    Cloning vectors are essential tools in biotechnology that facilitate the...

  6. 9.2.3
    Competent Host (For Transformation With Recombinant Dna)

    This section details the processes necessary to transform bacterial cells so...

  7. 9.3
    Processes Of Recombinant Dna Technology

    The section outlines the key processes involved in recombinant DNA...

  8. 9.3.1
    Isolation Of The Genetic Material (Dna)

    This section discusses the process of isolating DNA from various organisms,...

  9. 9.3.2
    Cutting Of Dna At Specific Locations

    This section discusses the cutting of DNA at specific locations using...

  10. 9.3.3
    Amplification Of Gene Of Interest Using Pcr

    PCR (Polymerase Chain Reaction) is a technique that amplifies a specific...

  11. 9.3.4
    Insertion Of Recombinant Dna Into The Host Cell/organism

    This section discusses methods for introducing recombinant DNA into host...

  12. 9.3.5
    Obtaining The Foreign Gene Product

    This section describes the process and significance of expressing foreign...

  13. 9.3.6
    Downstream Processing

    Downstream processing involves the series of steps necessary to prepare a...

What we have learnt

  • Biotechnology utilizes live organisms and their products for human needs.
  • Genetic engineering allows for targeted modifications in an organism's DNA.
  • Recombinant DNA technology involves various techniques including the use of restriction enzymes and cloning vectors.

Key Concepts

-- Genetic Engineering
Techniques to alter the chemistry of genetic material (DNA and RNA) to introduce changes in the phenotype of host organisms.
-- Bioprocess Engineering
Maintaining sterile conditions in chemical processes to enable the growth of desired microbes or eukaryotic cells for producing biotechnological products.
-- Recombinant DNA
DNA molecules formed by laboratory methods of genetic recombination to combine DNA from two different sources.
-- Restriction Enzymes
Enzymes that cut DNA at specific sequences, playing a vital role in genetic engineering.
-- Cloning Vector
A DNA molecule used to transport foreign genetic material into another cell, allowing for replication.

Additional Learning Materials

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