Tissue Culture

7.2.5.1 Tissue Culture

Description

Quick Overview

Tissue culture is a method used to grow new plants by taking tissue or cells from the growing tip of a plant and placing them in an artificial medium.

Standard

In tissue culture, plant cells are separated and cultured in a controlled environment, allowing for rapid division and growth into calluses. These calluses are treated with hormones to differentiate into plantlets which are then grown into mature plants. This method is important for producing disease-free plants efficiently.

Detailed

Tissue Culture

Tissue culture is an innovative agricultural technique that enables the growth of new plants from a specific tissue sample taken from the parent plant, usually from the growing tip. Once the cells are removed, they are placed in a carefully formulated artificial medium that provides the necessary nutrients and environment for rapid cell division. This process forms a callus, a mass of undifferentiated cells.

The next vital step involves transferring this callus to another medium supplemented with specific growth hormones that signal the cells to differentiate into various plant tissues. Eventually, these cells develop into small plantlets, which can be transferred to soil, where they grow into fully mature plants.

Tissue culture is widely utilized for the propagation of plants under disease-free conditions, significantly increasing the production of various ornamental plants and crops. It provides several advantages, including the ability to produce many genetically identical plants from a single parent, ensuring uniformity in desired traits, and accelerating the breeding process. As a result, tissue culture is an essential technique in modern agriculture that fosters efficient plant propagation.

Key Concepts

  • Tissue Culture: The method of growing plants from tissue in an artificial medium.

  • Callus Formation: The initial step where undifferentiated cells proliferate.

  • Hormonal Induction: The use of hormones to stimulate cell differentiation.

Memory Aids

🎡 Rhymes Time

  • In a lab, we take a clip, plant it right, watch it flip; hormones help it grow with flair, into new plants, beyond compare.

πŸ“– Fascinating Stories

  • Imagine a gardener who wants to multiply a favorite flower. Instead of waiting for seeds, they take a small part of the stem, place it in special soil, water it carefully, and soon see many flowers bloomβ€”magic through tissue culture!

🧠 Other Memory Gems

  • C.H.A.T.: Cells grow, Hormones added, Advances made, Transplant to soil.

🎯 Super Acronyms

T.C. - Tissue Culturing

  • Take
  • Cultivate
  • Differentiate
  • Create!

Examples

  • Example 1: Producing a large number of banana plants from a single parent using tissue culture.

  • Example 2: Advancements in the greenhouse industry through mass propagation of ornamental plants.

Glossary of Terms

  • Term: Tissue Culture

    Definition:

    A technique for growing and propagating plants by using tissue from the plant's growing tip in a controlled environment.

  • Term: Callus

    Definition:

    A mass of undifferentiated plant cells that forms in tissue culture before developing into plantlets.

  • Term: Hormones

    Definition:

    Chemical substances that regulate growth and differentiation in plants; used in tissue culture to stimulate plant development.