Practice Steps - 3.2 | Tools and Techniques in Genetic Engineering | Genetic Engineering Basic
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Practice Questions

Test your understanding with targeted questions related to the topic.

Question 1

Easy

What do restriction enzymes do?

💡 Hint: Think about the enzymes that 'restrict' or limit DNA.

Question 2

Easy

What does DNA ligase do?

💡 Hint: Remember: cutting and pasting!

Practice 4 more questions and get performance evaluation

Interactive Quizzes

Engage in quick quizzes to reinforce what you've learned and check your comprehension.

Question 1

What is the function of restriction enzymes?

  • Pick DNA sequences
  • Cut DNA at specific sites
  • Amplify DNA

💡 Hint: Think about what 'restriction' does to a sequence.

Question 2

True or False: DNA ligase can only join DNA fragments from the same organism.

  • True
  • False

💡 Hint: Consider what recombinant DNA means.

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Challenge Problems

Push your limits with challenges.

Question 1

If you have a DNA sequence cut by a restriction enzyme resulting in fragments 500bp, 200bp, and 300bp, how would gel electrophoresis visualize these fragments? Predict the order based on size.

💡 Hint: Remember, smaller pieces move faster in the gel!

Question 2

Given that restriction enzymes can create sticky ends or blunt ends, discuss the advantages of using sticky ends in DNA ligation. Why are they often preferred?

💡 Hint: Think about how puzzle pieces fit together—sticky ends are like pieces that have edges fitting into each other.

Challenge and get performance evaluation