ICSE Class 12 Biotechnology by Pavan | Practice Test to Test Your Knowledge
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ICSE Class 12 Biotechnology

ICSE Class 12 Biotechnology

Detailed mock test covering Business Economics, Financial Management, and Economic Environment. Includes case studies and real-world applications.

2025-07-20
Pavan Pavan
ICSE Class 12 Biotechnology Grade 12

Duration

30 min

Questions

20

Marking

Negative

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Questions Preview

Which of the following is a component of a nucleotide but not of a nucleoside?

A
Phosphate group
B
Nitrogenous base
C
Deoxyribose sugar
D
Ribose sugar

Chargaff's rule states that:

A
Purines = Pyrimidines
B
A = T and G = C
C
DNA is double-stranded
D
A = G and T = C

Which form of DNA is described in Watson and Crick’s double helix model?

A
A-DNA
B
B-DNA
C
Z-DNA
D
C-DNA

In the Hershey and Chase experiment, which radioactive isotope labeled the DNA?

A
35S
B
14C
C
32P
D
3H

Which enzyme is responsible for unwinding the DNA double helix?

A
Topoisomerase
B
Ligase
C
Helicase
D
Polymerase

Okazaki fragments are formed on which strand during DNA replication?

A
Leading strand
B
Lagging strand
C
Both strands
D
Template strand

Which enzyme joins Okazaki fragments during DNA replication?

A
Helicase
B
Primase
C
Ligase
D
Polymerase

What did the Meselson-Stahl experiment demonstrate?

A
DNA is conservative
B
DNA is semiconservative
C
DNA is dispersive
D
RNA is genetic material

Which base is found only in RNA and not in DNA?

A
Thymine
B
Uracil
C
Cytosine
D
Adenine

The B-form DNA has how many base pairs per turn?

A
10
B
11
C
12
D
9

Which enzyme synthesizes the RNA primer during DNA replication?

A
Helicase
B
Primase
C
Ligase
D
Topoisomerase

What is the primary function of DNA polymerase?

A
Break hydrogen bonds
B
Form peptide bonds
C
Add nucleotides to a growing DNA chain
D
Join Okazaki fragments

Topoisomerase prevents which of the following during replication?

A
Strand separation
B
DNA supercoiling
C
Base pairing
D
Primer synthesis

Which of the following statements about DNA replication is correct?

A
It proceeds in the 3’ to 5’ direction
B
It is conservative
C
It occurs in the cytoplasm
D
It requires DNA polymerase and primers

Which scientist(s) used Vicia faba to prove semiconservative replication?

A
Meselson and Stahl
B
Watson and Crick
C
Taylor et al.
D
Hershey and Chase

Which enzyme fills the gaps after removal of RNA primers?

A
DNA ligase
B
DNA polymerase I
C
Helicase
D
Topoisomerase

Which strand is synthesized continuously during replication?

A
Lagging strand
B
Leading strand
C
Template strand
D
Sense strand

Which enzyme in prokaryotes removes RNA primers?

A
DNA polymerase III
B
DNA polymerase I
C
Ligase
D
Primase

In which direction does DNA synthesis occur?

A
3’ to 5’
B
Randomly
C
5’ to 3’
D
Both directions simultaneously

What do SSB proteins do during DNA replication?

A
Synthesize RNA primers
B
Stabilize the unwound DNA
C
Join DNA fragments
D
Proofread newly synthesized DNA