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The study of microbiology encompasses the exploration of single-celled organisms, their diversity, and their vital roles in ecosystems and biotechnology. Key concepts include the difference between prokaryotic and eukaryotic organisms, the importance of species and strains, and methods for microorganism identification and classification. The principles of microscopy, ecological impacts of microorganisms, sterilization techniques, media compositions for culturing, and the kinetics of microbial growth are essential for applications in engineering and healthcare.