Practice - Spectrophotometry (Measuring Light Absorption)
Practice Questions
Test your understanding with targeted questions
What is the Beer-Lambert Law?
💡 Hint: Think about how concentration affects absorbance.
What does absorbance measure?
💡 Hint: Relate this to how light interacts with substances.
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Interactive Quizzes
Quick quizzes to reinforce your learning
What does the Beer-Lambert Law relate?
💡 Hint: Remember how concentration affects absorbance.
True or False: Absorbance can decrease if the concentration of a solution increases.
💡 Hint: Think about the definition of absorbance.
1 more question available
Challenge Problems
Push your limits with advanced challenges
If a solution has an absorbance of 0.25 at 340 nm and a path length of 1 cm with a molar extinction coefficient of 6220 M⁻¹ cm⁻¹, calculate the concentration of NADH.
💡 Hint: Identify the variables used in the formula.
Discuss how varying the path length might affect absorbance readings. What implications does this have for optimal experimental design?
💡 Hint: Consider how absorbance relates to concentration.
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