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Test your understanding with targeted questions related to the topic.
Question 1
Easy
What is the main purpose of monitoring enzyme-catalyzed reactions?
💡 Hint: Think about why knowing how fast a reaction occurs is important.
Question 2
Easy
Name one common technique used for monitoring enzyme activity.
💡 Hint: Which method helps measure light absorption?
Practice 4 more questions and get performance evaluation
Engage in quick quizzes to reinforce what you've learned and check your comprehension.
Question 1
What does monitoring enzyme-catalyzed reactions allow researchers to quantify?
💡 Hint: Think about the overall goal of studying enzyme activity.
Question 2
True or False: Fluorometry is less sensitive than spectrophotometry.
💡 Hint: Recall the sensitivity differences in measuring enzyme activity.
Solve 2 more questions and get performance evaluation
Push your limits with challenges.
Question 1
You are assessing the activity of an enzyme using spectrophotometry. The absorbance measured at 340 nm increased from 0.200 to 0.250 over 5 minutes. Calculate the initial reaction rate (V0).
💡 Hint: Remember to focus on the relationship between absorbance change and time.
Question 2
In a titration experiment designed to assess enzyme activity affecting pH, you observed that the pH dropped from 7.4 to 6.5 after enzyme addition. Convert this change into moles of acid produced if the buffer capacity is known to be 0.1 M.
💡 Hint: Consider how pH change correlates with acid concentration.
Challenge and get performance evaluation