Practice Spectrophotometry (Measuring Light Absorption) - 5.1.2.1 | Module 5: Enzymes – The Catalysts of Life | Biology (Biology for Engineers)
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5.1.2.1 - Spectrophotometry (Measuring Light Absorption)

Learning

Practice Questions

Test your understanding with targeted questions related to the topic.

Question 1

Easy

What is the Beer-Lambert Law?

💡 Hint: Think about how concentration affects absorbance.

Question 2

Easy

What does absorbance measure?

💡 Hint: Relate this to how light interacts with substances.

Practice 4 more questions and get performance evaluation

Interactive Quizzes

Engage in quick quizzes to reinforce what you've learned and check your comprehension.

Question 1

What does the Beer-Lambert Law relate?

  • Absorbance and temperature
  • Absorbance and concentration
  • Absorbance and pressure

💡 Hint: Remember how concentration affects absorbance.

Question 2

True or False: Absorbance can decrease if the concentration of a solution increases.

  • True
  • False

💡 Hint: Think about the definition of absorbance.

Solve 1 more question and get performance evaluation

Challenge Problems

Push your limits with challenges.

Question 1

If a solution has an absorbance of 0.25 at 340 nm and a path length of 1 cm with a molar extinction coefficient of 6220 M⁻¹ cm⁻¹, calculate the concentration of NADH.

💡 Hint: Identify the variables used in the formula.

Question 2

Discuss how varying the path length might affect absorbance readings. What implications does this have for optimal experimental design?

💡 Hint: Consider how absorbance relates to concentration.

Challenge and get performance evaluation