5.2 - Considerations
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Practice Questions
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What does host compatibility refer to in vector selection?
💡 Hint: Think about what it means for the vector to 'work'.
Why is insert size important when selecting a vector?
💡 Hint: Consider sizes of common vectors.
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Interactive Quizzes
Quick quizzes to reinforce your learning
What is the primary consideration when choosing a vector based on genome size?
💡 Hint: Which factor relates to the amount of DNA you can clone?
True or False: All vectors can be inserted into any host organism.
💡 Hint: Can you think of examples of compatible hosts?
2 more questions available
Challenge Problems
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Design an experiment using a BAC to clone a 100 kb gene. Describe the vector choice, expression level considerations, and how you would manage biosafety.
💡 Hint: Consider which elements are required for successful cloning.
Suppose researchers want to produce a high quantity of protein from their cloned gene; suggest a suitable vector type and promoter, explaining why.
💡 Hint: What factors enhance protein yield?
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