Genetic Engineering Advance | Advanced Molecular Biology Techniques in Genetic Engineering by Diljeet Singh | Learn Smarter
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Advanced Molecular Biology Techniques in Genetic Engineering

This chapter presents advanced molecular biology techniques essential for contemporary genetic engineering. It covers methodologies for DNA/RNA manipulation, including PCR, restriction enzymes, gene synthesis, and advanced cloning strategies. Additionally, it emphasizes the role of various enzymes and vector systems in achieving precise genetic modifications.

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Sections

  • 1

    Techniques In Genetic Engineering

    This section outlines the principles and applications of Polymerase Chain Reaction (PCR) and its variants, which are essential DNA amplification techniques in genetic engineering.

    Learning Practice

  • 1.1

    Description

    Learning

  • 1.2

    Learning Objectives

    This section outlines the essential learning objectives for understanding advanced molecular biology techniques in genetic engineering.

    Learning Practice

  • 2

    Polymerase Chain Reaction (Pcr) And Variants

    Learning

  • 2.1

    Technique Application

    This section discusses various DNA amplification techniques, including conventional PCR, RT-PCR, qPCR, and digital PCR, which are crucial for genetic analysis.

    Learning Practice

  • 2.2

    Key Concept

    This section discusses the principles and applications of polymerase chain reaction (PCR) and its variants.

    Learning Practice

  • 3

    Restriction Enzymes And Dna Ligation

    Learning

  • 3.1

    Restriction Enzymes (Endonucleases)

    Restriction enzymes are crucial tools in genetic engineering as they cut DNA at specific sequences, enabling gene cloning and manipulation.

    Learning Practice

  • 3.2

    Dna Ligase

    DNA ligase is an enzyme that joins DNA fragments by forming phosphodiester bonds, crucial in molecular cloning techniques.

    Learning Practice

  • 3.3

    Sticky Ends Vs. Blunt Ends

    The section compares sticky ends and blunt ends, highlighting their differences in DNA ligation efficiency and applications in genetic engineering.

    Learning Practice

  • 3.4

    Practical Use

    This section delves into the practical applications of genetic engineering techniques, focusing on gene cloning, synthesis, and modification.

    Learning Practice

  • 4

    Gene Synthesis And Site-Directed Mutagenesis

    Learning

  • 4.1

    De Novo Gene Synthesis

    De novo gene synthesis involves constructing DNA sequences from scratch using synthetic oligonucleotides.

    Learning Practice

  • 4.2

    Site-Directed Mutagenesis

    Site-Directed Mutagenesis (SDM) is a powerful technique used to introduce specific mutations into DNA to study gene function and protein structure.

    Learning Practice

  • 4.3

    Overlap Extension Pcr

    Overlap Extension PCR is a technique used to introduce insertions and specific mutations in DNA sequences through PCR amplification.

    Learning Practice

  • 5

    Advanced Cloning Vectors And Expression Systems

    This section discusses various advanced cloning vectors and expression systems that enhance gene manipulation capabilities in genetic engineering.

    Learning Practice

  • 5.1

    Vector Type Functionality

    This section explores different types of vectors used in genetic engineering, emphasizing their functionalities and applications in cloning and gene expression.

    Learning Practice

  • 5.2

    Considerations

    This section discusses the critical considerations in selecting advanced cloning vectors and expression systems for genetic engineering applications.

    Learning Practice

  • 6

    Recombinase-Based Systems

    Learning

  • 6.1

    Cre-Loxp And Flp-Frt

    Cre-LoxP and FLP-FRT systems are site-specific recombination tools used for conditional gene editing in molecular biology.

    Learning Practice

  • 6.2

    Useful In Generating Modifications

    Learning

  • 7

    Chapter Summary

    This section summarizes the essential techniques of advanced molecular biology for genetic engineering.

    Learning Practice

References

Chapter 1_ Advanced Molecular Biology Techniques in Genetic Engineering.pdf

Class Notes

Memorization

What we have learnt

  • Mastery of molecular techni...
  • PCR and its variants are us...
  • Restriction enzymes and lig...

Final Test

Revision Tests