3.1 - Restriction Enzymes (Endonucleases)
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Practice Questions
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What are restriction enzymes used for?
💡 Hint: What is their primary function?
What do sticky ends refer to?
💡 Hint: Think about the shape of the cut.
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Interactive Quizzes
Quick quizzes to reinforce your learning
What do restriction enzymes do?
💡 Hint: Think about the action these enzymes perform on DNA.
True or False: Sticky ends are easier to join than blunt ends.
💡 Hint: Consider the structural differences in the ends.
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Challenge Problems
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You need to clone a gene with a sticky end using EcoRI into a plasmid that has also been cut with EcoRI. Describe the steps you would take.
💡 Hint: Consider what other components might be required in the process.
Explain how you would resolve a situation where you accidentally used a blunt-end producing restriction enzyme instead of a sticky-end one. How might this affect the cloning efficiency?
💡 Hint: Think about how docking works with different cut types.
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