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Practice Questions
Test your understanding with targeted questions related to the topic.
Question 1
Easy
What are restriction enzymes used for?
💡 Hint: What is their primary function?
Question 2
Easy
What do sticky ends refer to?
💡 Hint: Think about the shape of the cut.
Practice 4 more questions and get performance evaluation
Interactive Quizzes
Engage in quick quizzes to reinforce what you've learned and check your comprehension.
Question 1
What do restriction enzymes do?
- They amplify DNA
- They cut DNA
- They ligate DNA
💡 Hint: Think about the action these enzymes perform on DNA.
Question 2
True or False: Sticky ends are easier to join than blunt ends.
- True
- False
💡 Hint: Consider the structural differences in the ends.
Solve 2 more questions and get performance evaluation
Challenge Problems
Push your limits with challenges.
Question 1
You need to clone a gene with a sticky end using EcoRI into a plasmid that has also been cut with EcoRI. Describe the steps you would take.
💡 Hint: Consider what other components might be required in the process.
Question 2
Explain how you would resolve a situation where you accidentally used a blunt-end producing restriction enzyme instead of a sticky-end one. How might this affect the cloning efficiency?
💡 Hint: Think about how docking works with different cut types.
Challenge and get performance evaluation