3.3 - Sticky ends vs. blunt ends
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Practice Questions
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What is a sticky end?
💡 Hint: Think of it as sticky tape that helps DNA bits stick together.
What is a blunt end?
💡 Hint: Think of a blunt end like cutting a piece of paper straight across.
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Interactive Quizzes
Quick quizzes to reinforce your learning
What type of ends do sticky ends have?
💡 Hint: Think about the stickiness these DNA ends provide.
Blunt ends are more efficient than sticky ends for ligation.
💡 Hint: Recall the comparison made in class regarding binding ability.
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Challenge Problems
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You have two DNA fragments: one with sticky ends and one with blunt ends. How would you go about successfully ligating each fragment? Discuss the steps and reasoning behind your choices.
💡 Hint: Consider the properties of the DNA ends and how they affect ligation.
Evaluate a scenario in genetic engineering where the choice of blunt ends over sticky ends provides a distinct advantage. Discuss the implications.
💡 Hint: Think about cases where sequence-specific integration is essential.
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