3.2 - DNA Ligase
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Practice Questions
Test your understanding with targeted questions
What is the role of DNA ligase?
💡 Hint: Think about what happens in DNA replication.
What type of ends do sticky ends have?
💡 Hint: How do they differ from blunt ends?
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Interactive Quizzes
Quick quizzes to reinforce your learning
What is the main function of DNA ligase?
💡 Hint: Consider what ligase means!
True or False: Sticky ends are more efficient in ligation than blunt ends.
💡 Hint: Think about their structure.
1 more question available
Challenge Problems
Push your limits with advanced challenges
Given a scenario where you have plasmids with sticky ends from EcoRI and blunt ends from another enzyme, explain how you would approach cloning a gene into a plasmid and why one method is preferred over the other.
💡 Hint: Consider the nature of ligation processes.
In a DNA ligation experiment, it's found that the ligation efficiency is very low. What factors could contribute to this, and how would you troubleshoot the problem?
💡 Hint: Review key components involved in the ligation process.
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