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Test your understanding with targeted questions related to the topic.
Question 1
Easy
What is the role of DNA ligase?
π‘ Hint: Think about what happens in DNA replication.
Question 2
Easy
What type of ends do sticky ends have?
π‘ Hint: How do they differ from blunt ends?
Practice 4 more questions and get performance evaluation
Engage in quick quizzes to reinforce what you've learned and check your comprehension.
Question 1
What is the main function of DNA ligase?
π‘ Hint: Consider what ligase means!
Question 2
True or False: Sticky ends are more efficient in ligation than blunt ends.
π‘ Hint: Think about their structure.
Solve 1 more question and get performance evaluation
Push your limits with challenges.
Question 1
Given a scenario where you have plasmids with sticky ends from EcoRI and blunt ends from another enzyme, explain how you would approach cloning a gene into a plasmid and why one method is preferred over the other.
π‘ Hint: Consider the nature of ligation processes.
Question 2
In a DNA ligation experiment, it's found that the ligation efficiency is very low. What factors could contribute to this, and how would you troubleshoot the problem?
π‘ Hint: Review key components involved in the ligation process.
Challenge and get performance evaluation