Genetic Engineering Advance | Genetic Engineering in Stem Cells and Regenerative Medicine by Diljeet Singh | Learn Smarter
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Genetic Engineering in Stem Cells and Regenerative Medicine

Genetic Engineering in Stem Cells and Regenerative Medicine

Genetic engineering significantly enhances the potential of stem cells in regenerative medicine, facilitating advancements in tissue repair and disease modeling. Stem cells, including embryonic, induced pluripotent, and adult stem cells, serve as crucial tools in research and therapeutic applications. The integration of genetic tools allows for precise manipulation and improvement of stem cell functions, while ethical considerations continue to shape the landscape of research and application in this field.

24 sections

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  1. 1
    Types Of Stem Cells

    This section describes the primary types of stem cells, including their...

  2. 1.1
    Embryonic Stem Cells (Escs)

    Embryonic stem cells (ESCs) are pluripotent cells derived from blastocysts...

  3. 1.2
    Induced Pluripotent Stem Cells (Ipscs)

    Induced pluripotent stem cells (iPSCs) are reprogrammed cells that can...

  4. 1.3
    Adult Stem Cells

    This section delves into adult stem cells, their characteristics, and their...

  5. 2
    Genetic Engineering Of Stem Cells

    This section discusses methods of genetic engineering used in stem cell...

  6. 2.1
    Transcription Factor Reprogramming

    Transcription factor reprogramming involves the use of specific proteins to...

  7. 2.2

    CRISPR/Cas9 is a revolutionary gene editing tool used to modify DNA in...

  8. 2.3
    Lentiviral And Aav Vectors

    This section highlights the role of lentiviral and AAV vectors in gene...

  9. 3
    Applications In Regenerative Medicine

    This section highlights the diverse applications of stem cells in...

  10. 3.1

    This section discusses the applications of genetic engineering and stem cell...

  11. 3.2

    This section discusses the application of stem cells in cardiology, focusing...

  12. 3.3

    This section discusses the applications of genetic engineering in orthopedic...

  13. 3.4
    Ophthalmology

    This section focuses on the use of stem cell therapies in ophthalmology,...

  14. 3.5
    Use Of Scaffolds, Biomaterials, And 3d Printing

    This section discusses how scaffolds, biomaterials, and 3D printing...

  15. 4
    Disease Modeling And Drug Screening

    This section discusses the role of patient-derived iPSCs in studying...

  16. 4.1
    Patient-Derived Ipscs

    This section discusses patient-derived induced pluripotent stem cells...

  17. 4.2
    High-Throughput Drug Testing

    High-throughput drug testing utilizes genetically engineered stem cell lines...

  18. 4.3
    Examples
  19. 5
    Ethical And Safety Concerns

    This section highlights the ethical and safety concerns associated with stem...

  20. 5.1
    Esc Research

    This section examines the ethical and safety concerns surrounding embryonic...

  21. 5.2
    Tumorigenicity

    Tumorigenicity refers to the potential for stem cells to form tumors after...

  22. 5.3
    Germline Editing Risk

    This section discusses the risks associated with germline editing in the...

  23. 5.4
    Informed Consent

    Informed consent is a crucial aspect of regenerative medicine, ensuring that...

  24. 6
    Chapter Summary

    The chapter addresses the role of genetic engineering in stem cell research...

What we have learnt

  • Different types of stem cells include embryonic, induced pluripotent, and adult stem cells, each with unique characteristics.
  • Genetic engineering techniques like CRISPR, transcription factor reprogramming, and viral vectors are pivotal to enhancing stem cell capabilities.
  • Regenerative medicine applications span various fields, including neurology, cardiology, and orthopedics, utilizing stem cells for effective tissue repair and regeneration.

Key Concepts

-- Embryonic Stem Cells (ESCs)
Pluripotent stem cells derived from blastocysts that can differentiate into any cell type in the body.
-- Induced Pluripotent Stem Cells (iPSCs)
Adult cells reprogrammed to an embryonic-like state, capable of differentiating into various cell types, helping to overcome ethical issues associated with ESCs.
-- CRISPR/Cas9
A revolutionary genetic editing tool that allows for precise alterations in DNA, enabling corrections of mutations in cells.
-- Tumorigenicity
The potential of stem cells to form tumors post-transplantation, which poses safety concerns in regenerative therapies.
-- Ethical Oversight
Regulatory framework needed to address moral concerns in stem cell research, especially surrounding the use of embryonic cells.

Additional Learning Materials

Supplementary resources to enhance your learning experience.