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Test your understanding with targeted questions related to the topic.
Question 1
Easy
What is the role of Cre recombinase in the Cre-LoxP system?
π‘ Hint: Think about the specific site it acts on.
Question 2
Easy
What is an FRT site used for?
π‘ Hint: What enzyme interacts with this site?
Practice 4 more questions and get performance evaluation
Engage in quick quizzes to reinforce what you've learned and check your comprehension.
Question 1
What type of sequences do Cre and FLP recognize?
π‘ Hint: These sequences are specific to each system.
Question 2
True or False: The Cre-LoxP system can be used without specific tissue targeting.
π‘ Hint: Consider how and when gene editing is applied.
Solve 2 more questions and get performance evaluation
Push your limits with challenges.
Question 1
Design a genetic experiment using the Cre-LoxP system to study a gene causing a hereditary disorder in mice. What considerations would you make regarding the timing and tissue specificity of Cre expression?
π‘ Hint: Think about the implications of gene activation timing.
Question 2
Explain how using both the Cre-LoxP and FLP-FRT systems in a single organism can provide insights into gene interaction and redundancy.
π‘ Hint: Consider what happens when you knock out multiple genes.
Challenge and get performance evaluation