Practice Guide RNA (gRNA) Design and PAM Sequences - 3 | CRISPR-Cas Systems and Genome Editing Tools | Genetic Engineering Advance
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Practice Questions

Test your understanding with targeted questions related to the topic.

Question 1

Easy

What does gRNA stand for?

πŸ’‘ Hint: Consider what it's guiding.

Question 2

Easy

What does PAM stand for?

πŸ’‘ Hint: Think about the role of PAM in CRISPR.

Practice 4 more questions and get performance evaluation

Interactive Quizzes

Engage in quick quizzes to reinforce what you've learned and check your comprehension.

Question 1

What is the length of a typical gRNA sequence?

  • 15 nt
  • 20 nt
  • 25 nt

πŸ’‘ Hint: Think about how specific you need to be in a targeting sequence.

Question 2

Is PAM necessary for the functioning of Cas enzymes?

  • True
  • False

πŸ’‘ Hint: Remember the role of locks and keys in binding.

Solve 2 more questions and get performance evaluation

Challenge Problems

Push your limits with challenges.

Question 1

Given a target DNA sequence of 'ACGTTAGC', suggest a potential gRNA sequence and explain its importance.

πŸ’‘ Hint: Ensure to analyze the area next to the target DNA for PAM correctness.

Question 2

Critically analyze how changes in either gRNA or PAM sequence could affect the CRISPR editing outcome.

πŸ’‘ Hint: Consider the Keystones of CRISPR: gRNA leads, PAM supports.

Challenge and get performance evaluation