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Practice Questions
Test your understanding with targeted questions related to the topic.
Question 1
Easy
What is the purpose of a calibration curve in quantitative fluorescence?
💡 Hint: Think about how we measure concentration from intensity.
Question 2
Easy
Define the inner filter effect.
💡 Hint: What happens at high concentrations?
Practice 4 more questions and get performance evaluation
Interactive Quizzes
Engage in quick quizzes to reinforce what you've learned and check your comprehension.
Question 1
What is a primary use of a calibration curve in fluorescence spectroscopy?
- To identify molecular structures
- To correlate intensity with concentration
- To quantify temperature variations
💡 Hint: Think about how calibration aligns with measuring concentration.
Question 2
True or False: The inner filter effect only occurs at low concentrations of analyte.
- True
- False
💡 Hint: Consider what happens to light absorption as concentration increases.
Solve 1 more question and get performance evaluation
Challenge Problems
Push your limits with challenges.
Question 1
A solution exhibits an absorbance of 0.15 at excitation and 0.10 at emission wavelengths. Calculate the corrected fluorescence intensity assuming a measured intensity of 0.70.
💡 Hint: Remember to average the absorbances.
Question 2
If the Stern–Volmer constant K_SV is found to be 1.2, with a quencher concentration of 0.05 M, calculate the ratio of intensities I₀/I.
💡 Hint: Revisit how to apply the Stern–Volmer equation.
Challenge and get performance evaluation