Practice Subculturing (passaging) (3.3.1) - Chapter 3: Cell Culture Technology
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Subculturing (Passaging)

Practice - Subculturing (Passaging)

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Practice Questions

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Question 1 Easy

What is subculturing?

💡 Hint: Think about why we need to do this in cell cultures.

Question 2 Easy

What technique is commonly used to detach adherent cells?

💡 Hint: What enzyme might come to mind?

4 more questions available

Interactive Quizzes

Quick quizzes to reinforce your learning

Question 1

What is the main purpose of subculturing cells?

To increase cell density
To maintain healthy growth
To produce more waste

💡 Hint: Consider the needs of cells in culture.

Question 2

True or False: Primary cells can be subcultured indefinitely.

True
False

💡 Hint: Think about the life span of the cells.

1 more question available

Challenge Problems

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Challenge 1 Hard

You are tasked with maintaining a cell line that shows signs of overconfluence. Describe the appropriate steps you would take to subculture these cells and ensure their optimal health.

💡 Hint: Focus on each step and the importance of sterility.

Challenge 2 Hard

During a routine subculture, you accidentally introduced a potential contaminant. What actions should you take to address the contamination?

💡 Hint: Think about contamination sources and how to mitigate them.

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