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Chapter 3: Cell Culture Technology

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Sections

  • 3

    Cell Culture Technology

    Cell culture technology encompasses the methods for growing cells in vitro, crucial for biotechnological applications, research, and pharmaceutical development.

    Learning Practice

  • 3.1

    Basics Of Cell Culture

    Cell culture involves growing cells in controlled environments, divided into primary and cell line cultures, and requires specific media and sterility techniques.

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  • 3.1.1

    Primary Cell Culture

    Primary cell culture involves the cultivation of cells directly isolated from tissues, which can be essential for various scientific and medical applications.

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  • 3.1.2

    Cell Line Culture

    Cell line culture involves the continuous growth of subcultured cells derived from primary cultures, crucial for various biotechnological applications.

    Learning Practice

  • 3.1.3

    Cell Culture Medium

    Cell culture mediums provide essential nutrients for the growth and maintenance of cells in vitro.

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  • 3.1.3.1

    Natural Media

    Natural media are culture media made from biological sources that offer essential nutrients for cell growth.

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  • 3.1.3.2

    Synthetic Media

    Synthetic media are chemically defined culture media that provide precise nutrient composition for cell growth, free from animal-derived products.

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  • 3.1.3.3

    Semi-Synthetic Media

    Semi-synthetic media combine natural and synthetic components for cell culture.

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  • 3.1.4

    Sterility And Aseptic Conditions

    Sterility and aseptic conditions are crucial in cell culture practices, ensuring that cultures remain free from microbial contamination.

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  • 3.2

    Types Of Cell Culture

    This section categorizes cell cultures based on their source, growth method, and applications.

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  • 3.2.1

    Adherent Cultures

    Adherent cultures are cell cultures that require a surface for attachment and growth, typically used for various biological and medical applications.

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  • 3.2.2

    Suspension Cultures

    Suspension cultures are cell cultures that grow freely in the culture medium, without the need for attachment to surfaces.

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  • 3.2.3

    Co-Culture Systems

    Co-culture systems involve the simultaneous growth of different types of cells to study their interactions and physiology.

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  • 3.2.4

    Embryonic And Adult Stem Cell Cultures

    This section discusses the unique properties of embryonic and adult stem cells, emphasizing their applications in medical research and therapy.

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  • 3.3

    Techniques In Cell Culture

    This section outlines key techniques essential for the successful growth and maintenance of cell cultures in biotechnological applications.

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  • 3.3.1

    Subculturing (Passaging)

    Subculturing, or passaging, is a vital technique used in cell culture to transfer cells to new culture vessels to ensure healthy growth and prevent overcrowding.

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  • 3.3.2

    Cell Counting

    Cell counting is a crucial technique that involves determining the number of viable cells in a culture using various methods.

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  • 3.3.3

    Cryopreservation

    Cryopreservation is a technique used to preserve cells by freezing them at very low temperatures to prevent cellular degradation.

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  • 3.3.4

    Cell Transformation

    Cell transformation involves altering the genetic material of a cell to enhance its growth properties or endow it with new characteristics.

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  • 3.3.5

    Bioreactors

    Bioreactors are crucial devices that provide controlled environments for the large-scale production of cell-based products.

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  • 3.4

    Applications Of Cell Culture Technology

    Cell culture technology has extensive applications across various fields, including pharmaceuticals, genetic engineering, regenerative medicine, cancer research, toxicity testing, and gene therapy.

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  • 3.4.1

    Pharmaceutical Production

    This section discusses how cell culture technology is integral to pharmaceutical production, detailing the production of vaccines and biopharmaceuticals.

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  • 3.4.2

    Genetic Engineering

    Genetic engineering involves the modification of an organism's DNA to produce desired traits or products.

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  • 3.4.3

    Regenerative Medicine

    Regenerative medicine utilizes cell culture technology, particularly stem cells, to engineer tissues and organs for transplantation and repair.

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  • 3.4.4

    Cancer Research

    The section discusses the significance of cell culture technology in cancer research, detailing how cultured cancer cells contribute to studying cancer biology, drug screening, and the development of new therapies.

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  • 3.4.5

    Toxicity Testing

    Toxicity testing involves using cell culture systems to assess the harmful effects of drugs, chemicals, and pollutants on living cells.

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  • 3.4.6

    Gene Therapy

    Gene therapy involves using cultured cells to deliver therapeutic genes to patients with genetic disorders, offering potential treatments for various illnesses.

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  • 3.5

    Challenges And Future Directions

    This section discusses the current challenges in cell culture technology and explores potential future advancements.

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  • 3.6

    Summary

    Cell culture technology enables the controlled growth of cells outside their natural environment, facilitating significant advancements in biotechnology.

    Learning Practice

References

12 Biotechnology ch3.pdf

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