Chapter 3: Cell Culture Technology

Sections

Navigate through the learning materials and practice exercises.

1. 3

   Cell Culture Technology

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   Cell culture technology encompasses the methods for growing cells in vitro,...

2. 3.1

   Basics Of Cell Culture

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   Cell culture involves growing cells in controlled environments, divided into...

3. 3.1.1

   Primary Cell Culture

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   Primary cell culture involves the cultivation of cells directly isolated...

4. 3.1.2

   Cell Line Culture

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   Cell line culture involves the continuous growth of subcultured cells...

5. 3.1.3

   Cell Culture Medium

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   Cell culture mediums provide essential nutrients for the growth and...

6. 3.1.3.1

   Natural Media

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   Natural media are culture media made from biological sources that offer...

7. 3.1.3.2

   Synthetic Media

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   Synthetic media are chemically defined culture media that provide precise...

8. 3.1.3.3

   Semi-Synthetic Media

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   Semi-synthetic media combine natural and synthetic components for cell culture.

9. 3.1.4

   Sterility And Aseptic Conditions

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   Sterility and aseptic conditions are crucial in cell culture practices,...

10. 3.2

    Types Of Cell Culture

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    This section categorizes cell cultures based on their source, growth method,...

11. 3.2.1

    Adherent Cultures

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    Adherent cultures are cell cultures that require a surface for attachment...

12. 3.2.2

    Suspension Cultures

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    Suspension cultures are cell cultures that grow freely in the culture...

13. 3.2.3

    Co-Culture Systems

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    Co-culture systems involve the simultaneous growth of different types of...

14. 3.2.4

    Embryonic And Adult Stem Cell Cultures

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    This section discusses the unique properties of embryonic and adult stem...

15. 3.3

    Techniques In Cell Culture

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    This section outlines key techniques essential for the successful growth and...

16. 3.3.1

    Subculturing (Passaging)

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    Subculturing, or passaging, is a vital technique used in cell culture to...

17. 3.3.2

    Cell Counting

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    Cell counting is a crucial technique that involves determining the number of...

18. 3.3.3

    Cryopreservation

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    Cryopreservation is a technique used to preserve cells by freezing them at...

19. 3.3.4

    Cell Transformation

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    Cell transformation involves altering the genetic material of a cell to...

20. 3.3.5

    Bioreactors

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    Bioreactors are crucial devices that provide controlled environments for the...

21. 3.4

    Applications Of Cell Culture Technology

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    Cell culture technology has extensive applications across various fields,...

22. 3.4.1

    Pharmaceutical Production

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    This section discusses how cell culture technology is integral to...

23. 3.4.2

    Genetic Engineering

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    Genetic engineering involves the modification of an organism's DNA to...

24. 3.4.3

    Regenerative Medicine

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    Regenerative medicine utilizes cell culture technology, particularly stem...

25. 3.4.4

    Cancer Research

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    The section discusses the significance of cell culture technology in cancer...

26. 3.4.5

    Toxicity Testing

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    Toxicity testing involves using cell culture systems to assess the harmful...

27. 3.4.6

    Gene Therapy

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    Gene therapy involves using cultured cells to deliver therapeutic genes to...

28. 3.5

    Challenges And Future Directions

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    This section discusses the current challenges in cell culture technology and...

29. 3.6

    Summary

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    Cell culture technology enables the controlled growth of cells outside their...