In humans, sex determination is governed by chromosomes found in cells, particularly the sex chromosomes, X and Y. Every human has 23 pairs of chromosomes, including one pair that determines sex. Females possess two X chromosomes (XX), while males have one X and one Y chromosome (XY). During fertilization, the unfertilized egg always carries one X chromosome. Sperm can carry either an X or a Y chromosome. If sperm with an X chromosome fertilizes the egg, the resulting zygote is female (XX). If sperm with a Y chromosome fertilizes the egg, the zygote is male (XY). Therefore, the father’s sperm chromosome determines the sex of the offspring, debunking the myth that the mother influences this outcome.