Environmental Quality Monitoring & Analysis, - Vol 2 | 15. Analysis Methods – Liquid Chromatography by Abraham | Learn Smarter
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15. Analysis Methods – Liquid Chromatography

Liquid chromatography (LC) offers significant advantages over gas chromatography (GC) in terms of sample handling and analysis. With the ability to manipulate solvent mixtures dynamically and maintain high detection sensitivity, LC is suitable for a broader range of compounds, particularly those that may denature during extraction. The chapter also discusses various detectors used in LC, emphasizing the role of UV-Vis and fluorescence spectroscopy in compound identification based on absorbance characteristics.

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Sections

  • 1

    Environmental Quality: Monitoring And Analysis

    This section explores liquid chromatography as a method of analysis in environmental quality monitoring, comparing it with gas chromatography and discussing its operational mechanics.

    Learning Practice

  • 1.1

    Prof. Ravi Krishna

    This section focuses on the principles and applications of liquid chromatography (LC), detailing its advantages over gas chromatography (GC) and the methods of detection involved.

    Learning Practice

  • 1.2

    Department Of Chemical Engineering

    Learning

  • 1.3

    Indian Institute Of Technology-Madras

    This section discusses liquid chromatography, its advantages over gas chromatography, and the associated analytical methods.

    Learning Practice

  • 1.4

    Lecture No. 27

    This section discusses liquid chromatography as a method for analyzing environmental quality, highlighting its advantages over gas chromatography.

    Learning Practice

  • 1.5

    Analysis Methods – Liquid Chromatography

    This section discusses the operational principles and advantages of liquid chromatography (LC), particularly in contrast to gas chromatography (GC).

    Learning Practice

  • 1.6

    Liquid Chromatography Overview

    Liquid chromatography (LC) is a method used for analyzing compounds that cannot be efficiently vaporized, allowing for better analysis of a wider range of samples compared to gas chromatography (GC).

    Learning Practice

  • 1.7

    Mobile Phase In Liquid Chromatography

    Liquid chromatography (LC) utilizes a liquid mobile phase to separate compounds based on their interactions with a stationary phase.

    Learning Practice

  • 1.8

    Detectors In Liquid Chromatography

    This section discusses the use and functioning of various detectors in liquid chromatography, emphasizing the advantages of liquid chromatography over gas chromatography.

    Learning Practice

  • 1.9

    Uv Absorbance In Spectroscopy

    This section explains UV absorbance and its significance in spectroscopy, detailing how absorbance relates to concentration and detecting compounds.

    Learning Practice

References

27.pdf

Class Notes

Memorization

What we have learnt

  • Liquid chromatography is ea...
  • The mobile phase in LC can ...
  • Detectors commonly used in ...

Final Test

Revision Tests