15. Analysis Methods – Liquid Chromatography
Liquid chromatography (LC) offers significant advantages over gas chromatography (GC) in terms of sample handling and analysis. With the ability to manipulate solvent mixtures dynamically and maintain high detection sensitivity, LC is suitable for a broader range of compounds, particularly those that may denature during extraction. The chapter also discusses various detectors used in LC, emphasizing the role of UV-Vis and fluorescence spectroscopy in compound identification based on absorbance characteristics.
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1.2Department Of Chemical Engineering
What we have learnt
- Liquid chromatography is easier to operate compared to gas chromatography due to the absence of phase transfer issues.
- The mobile phase in LC can be a single solvent or a mixture, offering flexibility in solvent composition.
- Detectors commonly used in liquid chromatography include UV-Vis, refractive index, and fluorescence spectroscopy, which analyze the light absorption characteristics of compounds.
Key Concepts
- -- Liquid Chromatography (LC)
- A separation technique that uses a liquid mobile phase to separate compounds within a mixture based on their affinity to a stationary phase.
- -- UVVis Spectroscopy
- A technique used in liquid chromatography to measure the absorbance of UV light by a sample, allowing for the quantification of substance concentration.
- -- Mobile Phase
- The solvent or mixture of solvents in which the sample is dissolved in liquid chromatography, facilitating the movement of compounds through the column.
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