5.2. Dye Staining and Fluorescence Microscopy

Try these first

1. 1.

   What is the permissible limit of pathogens in water according to CPCB standards?

   Hint

   Look for regulations related to water quality.

2. 2.

   What does CFU stand for?

   Hint

   Remember the measure used for viable bacteria.

3. 3.

   According to CPCB standards, how many pathogens are acceptable in 100 ml of water?

   • 10
   • 100
   • 5

   Hint

   Think about the standard number mentioned in microbiology.

4. 4.

   True or False: Flow cytometry is the standard method for counting bacteria.

   • True
   • False

   Hint

   Consider the challenges discussed regarding flow cytometry.

5. 5.

   A water sample shows high turbidity. You suspect a high microbial presence. Outline the steps you would take to confirm the viability and types of microorganisms present.

   Hint

   Think about the importance of both quantitative and qualitative analysis.

6. 6.

   You notice that certain cultures yield many colonies that are difficult to distinguish. How would you modify your approach for clearer results in such scenarios?

   Hint

   Remember about the role of dilution in microbial analysis.