1.6. Monitoring and Measurement of Microorganisms

Try these first

1. 1.

   What is the CPCB standard for microorganisms in drinking water?

   Hint

   Think about the maximum acceptable level set by CPCB.

2. 2.

   What is a colony-forming unit (CFU)?

   Hint

   Consider what happens when bacteria grow on a nutrient medium.

3. 3.

   What is the acceptable level of microorganisms set by CPCB for drinking water?

   • 1 per 100 ml
   • 5 per 100 ml
   • 10 per 100 ml

   Hint

   Recall the exact figure provided in class.

4. 4.

   True or False: Dilution is not necessary when counting high concentrations of bacteria.

   • True
   • False

   Hint

   Consider why we dilute a sample.

5. 5.

   Design an experiment to measure the concentration of microorganisms in a lake sample, detailing each method and the rationale behind your choices.

   Hint

   Consider the steps we discussed and the importance of multiple analyses.

6. 6.

   Compare and contrast traditional culturing techniques with flow cytometry in terms of efficacy, speed, and application.

   Hint

   Focus on how each technique addresses challenges in microbial analysis.