Genetic Engineering Basic | Recombinant DNA Technology by Diljeet Singh | Learn Smarter
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Recombinant DNA Technology

Recombinant DNA technology is a key aspect of genetic engineering, combining DNA from various sources to produce new genetic configurations. This innovative process includes isolation of desired genes, their insertion into vectors, and transformation into host organisms, which can lead to significant applications in medicine, agriculture, and industry.

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Sections

  • 4

    Recombinant Dna Technology

    Recombinant DNA technology involves combining DNA from different sources to create new genetic combinations, essential for advancements in biotechnology.

    Learning Practice

  • 4.1

    What Is Recombinant Dna?

    Recombinant DNA (rDNA) is DNA formed by combining genetic material from different sources, key to genetic engineering.

    Learning Practice

  • 4.2

    Steps In Creating Recombinant Dna

    This section outlines the steps involved in creating recombinant DNA, highlighting key processes including gene isolation, cutting, insertion into vectors, and transformation into host cells.

    Learning Practice

  • 4.2.1

    Isolation Of Desired Gene

    This section discusses the process of isolating a desired gene from a DNA source, a critical step in recombinant DNA technology.

    Learning Practice

  • 4.2.2

    Cutting Dna With Restriction Enzymes

    Restriction enzymes are crucial tools in recombinant DNA technology that cut DNA at specific sequences, enabling gene manipulation.

    Learning Practice

  • 4.2.3

    Inserting Into A Vector

    Inserting a gene into a vector is crucial in the recombinant DNA technology process as it prepares the gene for introduction into a host organism.

    Learning Practice

  • 4.2.4

    Transfer Into Host Cell

    This section discusses the transfer of recombinant DNA into host cells, outlining the crucial step in genetic engineering.

    Learning Practice

  • 4.2.5

    Selection And Screening

    This section covers the process of selection and screening in recombinant DNA technology, emphasizing how scientists identify successful transformations of host cells with new genetic material.

    Learning Practice

  • 4.2.6

    Expression Of Gene

    The expression of a gene involves the transcription and translation of an inserted gene in a host organism, resulting in the production of a desired protein.

    Learning Practice

  • 4.3

    Gene Cloning

    Gene cloning involves creating multiple identical copies of a specific gene through recombinant DNA technology.

    Learning Practice

  • 4.4

    Applications Of Recombinant Dna Technology

    Recombinant DNA technology has numerous applications across medical, agricultural, and industrial fields, allowing for advancements such as genetically engineered organisms and biopharmaceuticals.

    Learning Practice

  • 4.4.1

    Medical Applications

    Recombinant DNA technology plays a pivotal role in medical advancements such as insulin production and gene therapy.

    Learning Practice

  • 4.4.2

    Agricultural Applications

    This section discusses how recombinant DNA technology is utilized in agriculture to create genetically modified organisms (GMOs) that enhance crop traits.

    Learning Practice

  • 4.4.3

    Industrial Applications

    This section discusses the industrial applications of recombinant DNA technology, highlighting how engineered microorganisms are utilized in various sectors.

    Learning Practice

  • 4.5

    Genetically Modified Organisms (Gmos)

    This section defines genetically modified organisms (GMOs) and provides examples of various GMOs produced using recombinant DNA technology.

    Learning Practice

References

Chapter 4_ Recombinant DNA Technology.pdf

Class Notes

Memorization

What we have learnt

  • Recombinant DNA is formed b...
  • The creation of recombinant...
  • Gene cloning facilitates th...

Revision Tests