11. Analysis Methods – Chromatography Fundamentals
The chapter provides an in-depth analysis of chromatography fundamentals, focusing on the separation processes of organic chemicals in environmental samples. It highlights the significance of considering multiple analytes present in a given sample and explains the role of relative affinity in separating these compounds. The discussion includes practical aspects of chromatographic methods, including both liquid and gas chromatography, emphasizing the importance of the stationary and mobile phases in sample analysis.
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What we have learnt
- Chromatography is primarily a separation technique rather than an analysis method.
- The process relies on the relative affinity of analytes to stationary and mobile phases for effective separation.
- Different types of chromatography, such as liquid and gas chromatography, serve distinct purposes in environmental analysis.
Key Concepts
- -- Chromatography
- A technique used to separate mixtures based on the differential affinities of the components to stationary and mobile phases.
- -- Stationary Phase
- The phase that does not move in chromatography, which interacts with the analytes to facilitate separation.
- -- Mobile Phase
- The phase that moves through the stationary phase, carrying the analytes with it.
- -- Relative Affinity
- The tendency of an analyte to interact with one phase over another, impacting its movement during chromatography.
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