Environmental Quality Monitoring & Analysis, - Vol 2 | 14. Chromatography and Calibration by Abraham | Learn Smarter
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14. Chromatography and Calibration

The chapter discusses the use of chromatography and mass spectrometry in quantitative and qualitative analysis of compounds. It highlights the importance of calibration based on concentration and the detection limits of different operational modes in GCMS, such as SCAN and SIM, emphasizing their impacts on sensitivity and the identification of compounds. The chapter also covers the significance of understanding peak integration and baseline determination in chromatograms for accurate analysis.

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Sections

  • 1

    Chromatography And Calibration

    This section discusses the principles of chromatography and the importance of calibration in quantitative analysis.

    Learning Practice

  • 1.1

    Area Under The Peak

    This section discusses the techniques and considerations involved in measuring the area under peaks in chromatograms, emphasizing the importance of calibration and concentration in quantitative analysis.

    Learning Practice

  • 1.2

    Calibration Procedures

    This section discusses the key aspects of calibration procedures in chromatographic analysis, focusing on concentration calibration and its implications for quantitative analysis.

    Learning Practice

  • 1.3

    Concentration Vs Mass

    This section explores the distinction between concentration and mass in the context of chemical analysis, specifically focusing on gas chromatography and the importance of calibration methods.

    Learning Practice

  • 1.4

    Calibration Curve And Errors

    This section discusses the importance of calibration curves in chromatography and the need to manage errors accurately to ensure valid quantification results.

    Learning Practice

  • 1.5

    Sensitivity And Detection

    This section discusses the principles of sensitivity and detection in chromatography, focusing on the calibration methods and their implications on quantitative analysis.

    Learning Practice

  • 1.6

    Selected Ion Monitoring (Sim)

    This section discusses Selected Ion Monitoring (SIM), a technique used in mass spectrometry to enhance the sensitivity of detecting specific compounds in a sample, emphasizing its operational distinctions from scanning methods.

    Learning Practice

  • 2

    Challenges In Organic Analysis

    This section discusses various challenges encountered in organic analysis, particularly in gas chromatography and mass spectrometry.

    Learning Practice

  • 2.1

    Detecting Low Concentrations

    This section discusses the methods and challenges associated with detecting low concentrations of compounds in chromatographic analysis.

    Learning Practice

  • 2.2

    Objective Setting For Analysis

    The section discusses the importance of setting clear objectives and calibration methods for effective chromatographic analysis, emphasizing sensitivity and quantification.

    Learning Practice

  • 2.3

    Derivatization

    This section discusses the concept of derivatization in the context of chromatography, emphasizing its importance for increasing the stability and detectability of analytes during analysis.

    Learning Practice

  • 3

    Quantitative And Qualitative Analysis

    This section discusses how quantitative and qualitative analyses are performed using chromatographic techniques, focusing on calibration, detection limits, and the importance of concentration measurements.

    Learning Practice

References

26b.pdf

Class Notes

Memorization

What we have learnt

  • Calibration in chromatograp...
  • Selected Ion Monitoring (SI...
  • The understanding of noise ...

Final Test

Revision Tests