14. Chromatography and Calibration
The chapter discusses the use of chromatography and mass spectrometry in quantitative and qualitative analysis of compounds. It highlights the importance of calibration based on concentration and the detection limits of different operational modes in GCMS, such as SCAN and SIM, emphasizing their impacts on sensitivity and the identification of compounds. The chapter also covers the significance of understanding peak integration and baseline determination in chromatograms for accurate analysis.
Enroll to start learning
You've not yet enrolled in this course. Please enroll for free to listen to audio lessons, classroom podcasts and take practice test.
Sections
Navigate through the learning materials and practice exercises.
What we have learnt
- Calibration in chromatography is essential for accurate concentration measurement.
- Selected Ion Monitoring (SIM) enhances sensitivity at the expense of broad identification capability.
- The understanding of noise versus signal is important for effective analysis in mass spectrometry.
Key Concepts
- -- Calibration
- The process of establishing a relationship between response and concentration in chromatography for accurate quantification.
- -- Selected Ion Monitoring (SIM)
- A GCMS operational mode that focuses on specific ions to increase detection sensitivity, often at the cost of comprehensive identification.
- -- Minimum Detection Limit (MDL)
- The lowest concentration of a substance that can be reliably detected by an analytical method.
- -- Peak Integration
- The calculation of the areas under peaks in a chromatogram to quantify compounds based on their concentration.
- -- Noise
- Unwanted electrical signals in data that can interfere with the analysis of a substance’s actual signal in chromatography and mass spectrometry.
Additional Learning Materials
Supplementary resources to enhance your learning experience.