In the Calvin Cycle, carbon dioxide (CO2) is fixed into organic compounds, and the first step of this process involves the reaction of CO2 with ribulose bisphosphate (RuBP), a 5-carbon ketose sugar. This reaction is catalyzed by the enzyme ribulose bisphosphate carboxylase/oxygenase (RuBisCO), resulting in the formation of two molecules of 3-phosphoglycerate (3-PGA), which are 3-carbon compounds. The discovery that RuBP is the primary acceptor molecule for CO2 was unexpected, as scientists initially believed that a 2-carbon compound would serve this role. Understanding this process is essential for grasping how plants convert inorganic carbon into organic matter, thereby supporting life on Earth.